Dwayne Mattis, Ian Breckin and Jack Hunt have a virus.
Mattis and Breckin missed the home league defeat to Oxford in midweek but Hunt played.
Kelvin Lomax came through a late fitness test to play on Tuesday night but suffered an ankle injury towards the end of the game and a late decision will be made on his fitness.
With Scott Griffiths back at Peterborough and Aaron Downes still a way off fitness following a long-term knee injury, boss John Sheridan has a threadbare backline.
Midfielder Derek Niven will complete a three-match suspension following his controversial sending-off in the league meeting between the two sides a fortnight ago but Craig Clay and Tendayi Darikwa are available again after their loan spells at Barrow.